nectin 2 pe (Miltenyi Biotec)
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Structured Review
Miltenyi Biotec
nectin 2 pe
Nectin 2 Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nectin+2+pe/CD112+Antibody%2C+anti-human%2C+REAfinity/pmc12901471-67-27-33
Average 94 stars, based on 7 article reviews
Nectin 2 Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nectin+2+pe/CD112+Antibody%2C+anti-human%2C+REAfinity/pmc12901471-67-27-33
Average 94 stars, based on 7 article reviews
nectin 2 pe - by Bioz Stars,
2026-10
94/100 stars
Images
1) Product Images from "Osteoclasts affect the anti-cancer activity of NK cells"
Article Title: Osteoclasts affect the anti-cancer activity of NK cells
Journal: Frontiers in Immunology
doi: 10.3389/fimmu.2026.1730283
Figure Legend Snippet: Expression of NK cell receptor ligands by OCs by flow cytometry. The graph shows the expression levels of PVR and Nectin-2, respectively ligands of DNAM-1 and TIGIT on OCs and on OC+NK cells after 3 days (A) . Characterization of NID-1 ligand of NKp44 and B7H6 ligand of NKp30 on OCs and on OCs+NK cells, with A549s and with both NK cells+A549s (B) . N = 6 experiments. Groups were compared using a one-way repeated measures analysis of variance with post hoc Tukey test (* p < 0.05; ** p < 0.01).
Techniques Used: Expressing, Flow Cytometry
Figure Legend Snippet: Analysis of CD133 + /CXCR4 + CSC subset. Evaluation of the CSC CD133 + /CXCR4 + subset of A549s, in co-culture with NK cells, OCs and with both OCs and NK cells (A) . Evaluation of expression levels of Nectin-2 (ligand of TIGIT and DNAM-1) (B) and Nectin-4 (ligand of TIGIT) (C) on CD133 + cells, in co-culture with NK cells, OCs and with NK cells+OCs (B, C) . N = 5 experiments. Groups were compared using a one-way repeated measures analysis of variance with post hoc Tukey test (* p < 0.05; ** p < 0.01).
Techniques Used: Co-Culture Assay, Expressing
Related Articles
Flow Cytometry:Article Title: Osteoclasts affect the anti-cancer activity of NK cells Article Snippet: To obtain CSCs (A549s), A549 cells were detached by Tripsin (Euroclone, Pero, Milano), counted and plated at 10.000 cells/well in Ultra-Low Attachment 6-multi-well (Thermo Fisher Scientific, Carlsbad, CA, USA), in a serum-free stem cell medium (SCM), constituted by DMEM/F12K (Gibco, Billings, MT, USA), supplemented with B27 (Gibco, Billings, MT, USA), EGF 20 ng/mL (Sigma Aldrich, Burlington, MA, USA), bFGF10 ng/mL (PeproTech, East Windsor, NJ, USA), Heparin 2 g/mL (Stemcell Technologies, Vancouver, Canada), and 1% Penicillin-Streptomycin. .. After 10 days, spheroid-like structures were originated, then dissociated by ACCUTASE and analyzed by flow cytometry through the staining with the following anti-human monoclonal antibodies CD133 PE-Vio770, Article Title: Osteoclasts affect the anti-cancer activity of NK cells Article Snippet: To obtain CSCs (A549s), A549 cells were detached by Tripsin (Euroclone, Pero, Milano), counted and plated at 10.000 cells/well in Ultra-Low Attachment 6-multi-well (Thermo Fisher Scientific, Carlsbad, CA, USA), in a serum-free stem cell medium (SCM), constituted by DMEM/F12K (Gibco, Billings, MT, USA), supplemented with B27 (Gibco, Billings, MT, USA), EGF 20 ng/mL (Sigma Aldrich, Burlington, MA, USA), bFGF10 ng/mL (PeproTech, East Windsor, NJ, USA), Heparin 2 g/mL (Stemcell Technologies, Vancouver, Canada), and 1% PenicillinStreptomycin. .. After 10 days, spheroid-like structures were originated, then dissociated by ACCUTASE and analyzed by flow cytometry through the staining with the following anti-human monoclonal antibodies CD133 PE-Vio770, Staining:Article Title: Osteoclasts affect the anti-cancer activity of NK cells Article Snippet: To obtain CSCs (A549s), A549 cells were detached by Tripsin (Euroclone, Pero, Milano), counted and plated at 10.000 cells/well in Ultra-Low Attachment 6-multi-well (Thermo Fisher Scientific, Carlsbad, CA, USA), in a serum-free stem cell medium (SCM), constituted by DMEM/F12K (Gibco, Billings, MT, USA), supplemented with B27 (Gibco, Billings, MT, USA), EGF 20 ng/mL (Sigma Aldrich, Burlington, MA, USA), bFGF10 ng/mL (PeproTech, East Windsor, NJ, USA), Heparin 2 g/mL (Stemcell Technologies, Vancouver, Canada), and 1% Penicillin-Streptomycin. .. After 10 days, spheroid-like structures were originated, then dissociated by ACCUTASE and analyzed by flow cytometry through the staining with the following anti-human monoclonal antibodies CD133 PE-Vio770, Article Title: Adoptive NK Cell Transfer as a Treatment in Colorectal Cancer Patients: Analyses of Tumour Cell Determinants Correlating With Efficacy In Vitro and In Vivo Article Snippet: .. The following antibodies were used for cell surface staining: ICAM-1-APC (clone HA58; BD, San Jose, CA, USA), HLA-ABC-FITC (clone W6/32; eBioscience, San Diego, CA, USA), HLA-E-PE (clone REA1031; Miltenyi), HLA-G-PerCPVio700 (clone 87G; Miltenyi), HLA-II-APCVio700 (REA332; Miltenyi), MICA/B-VioBright515 (clone REA1076; Miltenyi), PVR-PE (clone PV404.19; Miltenyi), Article Title: Osteoclasts affect the anti-cancer activity of NK cells Article Snippet: To obtain CSCs (A549s), A549 cells were detached by Tripsin (Euroclone, Pero, Milano), counted and plated at 10.000 cells/well in Ultra-Low Attachment 6-multi-well (Thermo Fisher Scientific, Carlsbad, CA, USA), in a serum-free stem cell medium (SCM), constituted by DMEM/F12K (Gibco, Billings, MT, USA), supplemented with B27 (Gibco, Billings, MT, USA), EGF 20 ng/mL (Sigma Aldrich, Burlington, MA, USA), bFGF10 ng/mL (PeproTech, East Windsor, NJ, USA), Heparin 2 g/mL (Stemcell Technologies, Vancouver, Canada), and 1% PenicillinStreptomycin. .. After 10 days, spheroid-like structures were originated, then dissociated by ACCUTASE and analyzed by flow cytometry through the staining with the following anti-human monoclonal antibodies CD133 PE-Vio770, Bioprocessing:Article Title: Osteoclasts affect the anti-cancer activity of NK cells Article Snippet: To obtain CSCs (A549s), A549 cells were detached by Tripsin (Euroclone, Pero, Milano), counted and plated at 10.000 cells/well in Ultra-Low Attachment 6-multi-well (Thermo Fisher Scientific, Carlsbad, CA, USA), in a serum-free stem cell medium (SCM), constituted by DMEM/F12K (Gibco, Billings, MT, USA), supplemented with B27 (Gibco, Billings, MT, USA), EGF 20 ng/mL (Sigma Aldrich, Burlington, MA, USA), bFGF10 ng/mL (PeproTech, East Windsor, NJ, USA), Heparin 2 g/mL (Stemcell Technologies, Vancouver, Canada), and 1% Penicillin-Streptomycin. .. After 10 days, spheroid-like structures were originated, then dissociated by ACCUTASE and analyzed by flow cytometry through the staining with the following anti-human monoclonal antibodies CD133 PE-Vio770, Article Title: Osteoclasts affect the anti-cancer activity of NK cells Article Snippet: To obtain CSCs (A549s), A549 cells were detached by Tripsin (Euroclone, Pero, Milano), counted and plated at 10.000 cells/well in Ultra-Low Attachment 6-multi-well (Thermo Fisher Scientific, Carlsbad, CA, USA), in a serum-free stem cell medium (SCM), constituted by DMEM/F12K (Gibco, Billings, MT, USA), supplemented with B27 (Gibco, Billings, MT, USA), EGF 20 ng/mL (Sigma Aldrich, Burlington, MA, USA), bFGF10 ng/mL (PeproTech, East Windsor, NJ, USA), Heparin 2 g/mL (Stemcell Technologies, Vancouver, Canada), and 1% PenicillinStreptomycin. .. After 10 days, spheroid-like structures were originated, then dissociated by ACCUTASE and analyzed by flow cytometry through the staining with the following anti-human monoclonal antibodies CD133 PE-Vio770, |